国产欧美一区二区精品仙草咪-亚洲bbw性色大片-亚洲2017天堂色无码-日韩精品人妻系列无码专区-亚洲高清无码视频网站在线

全國咨詢熱線:028-82610909

Ultrasound-assisted enzyme extraction of Dendrobium Officinale polysaccharides: Extraction process, characterization, immunomodulatory effects

發布時間:1738736547 人氣: 來源:

期刊名:Ultrasonics Sonochemistry

發表日期: Accepted 25 January 2025

文章地址:https://doi.org/10.1016/j.ultsonch.2025.107248


Abstract


Dendrobium Officinale is a traditional Chinese medicinal and edible herb, and polysaccharides being its principal active components. We employed ultrasonic-assisted enzymatic extraction to obtain polysaccharides from Dendrobium Officinale (DOP) and optimized the extraction process using the GA-BP method. Subsequently, DOP was characterized using HPLC, SEM, and HPGPC. Finally, an immunocompromised mouse model induced by cyclophosphamide was adopted to evaluate the immunomodulatory effects. The results showed that the optimal extraction process of ultrasound-assisted enzyme method was 102 min ultrasonic extraction time, 48 °C extraction temperature, 1:80 material-liquid ratio, and 1600 U/g enzyme additions. The predicted polysaccharide yield was 29.64 %, and the actual polysaccharide yield was 29.71 ± 0.31 %. DOP is a white powder with pore-like structure, whose polysaccharide content is 90.66 ± 2.47 %, and with a composition of D-mannose, glucose, galactose, arabinose in a molar ratio of 74.17: 47.80: 9.03: 1. DOP has demonstrated the ability to improve the general condition of mice with cyclophosphamide-induced immunosuppression. It mitigates damage to the thymus and spleen tissues, increases white blood cell counts, regulates the ratio of T lymphocytes and the levels of related factors, enhances macrophage phagocytic function to improve nonspecific immune responses, and boosts the levels of immunoglobulins IgA and IgG to enhance specific immune function. Therefore, DOP can alleviate immunosuppression induced by cyclophosphamide.

2. Materials and methods

2.1. Materials and chemicals


The Dendrobium Officinale used in the experiments was identified as the dried stem of Dendrobium Officinale Kimura & Migo by Xiaona Yu (Chief Pharmacist of Chinese Medicine at Shaoxing Central Hospital), and purchased from Zhejiang Yunzhitang Biotechnology Co., LTD. Cellulase (50 U/mg) was purchased from Shanghai Yuanye Biotechnology Co., LTD (Shanghai, China). IgA, IgG, IL-1, IL-6, and IL-10 ELISA kits were purchased from Jiangsu Meimian Industrial Co., LTD (Jiangsu, China). CD3+, CD4+, CD8+ antibodies were purchased from Multisciences (LianKe) Biotech, CO., LTD (Zhejiang, China). Reference standards were purchased from Chengdu Pufei De Biotech CO., LTD (Sichuan, China). CD4, CD8 antibody were purchased from Daige Biotechnology Co., Ltd (Zhejiang, China). All the additional chemicals were of analytical quality or above, and all water used for separation and examination was filtered and deionized.


短小蛇根草苷|126722-26-7
白花蛇舌草苷分析對照品 80159-07-5
異白鮮堿|484-74-2

在線留言

在線客服
聯系方式

熱線電話

028-82610909

上班時間

周一到周五

公司電話

028-82610909

二維碼
主站蜘蛛池模板: 尤物视频在线观看| av无码岛国免费动作片| 一区二区三区四区在线 | 欧洲| 人人妻人人澡av天堂香蕉| 欧美成人精品第一区| 国产精品无码无需播放器| 国产精品久久久久久tv| 亚洲一区av无码专区在线观看| 天天狠天天天天透在线| 国产精品爽爽ⅴa在线观看| 5d肉蒲团之性战奶水欧美| 四虎国产精品免费久久| 宅男色影视亚洲人在线| 国产乱妇无乱码大黄aa片| 亚洲久悠悠色悠在线播放| 超薄肉色丝袜一区二区| 亚洲综合av色婷婷国产野外| 国产色视频一区二区三区| 免费无码av一区二区三区| 国产精品午夜无码av体验区| 精品无人区麻豆乱码1区2区新区| 高清自拍亚洲精品二区| 国产人与禽zoz0性伦多活几年| 亚洲成av人片在线观看| 人妻少妇456在线视频| 亚洲精品国产成人精品| 久久国产一区二区三区| 国产成人8x人在线视频软件| 日本九九热在线观看官网| 97色伦97色伦国产| 久久精品水蜜桃av综合天堂| 尤物国产在线精品一区| 国产精品久久久久久久9999| 日韩精品国产另类专区| 久久香综合精品久久伊人| 国产免费视频| 精品国产一区二区三区四区五区| 成人国产片视频在线观看| 秋霞鲁丝片一区二区三区| 色婷婷色综合激情国产日韩| 男人女人做爽爽18禁网站|